Nowadays renewable energy sources are given great consideration. This is determined by the following key factors: aim to ensure energy supply and mitigate dangerous climate change associated with greenhouse gas emissions into the atmosphere. This paper presents the environmental impact in used car tyres life cycle. “Gabi 6” software was used for environmental impact assessment. The four environmental impact categories were evaluated: global warming potential for 100 years, acidification potential, eutrophication potential and ozone layer depletion potential. Results show that even if biofuels are cleaner than usual fossil fuel, production of biofuels make a significant environmental impact.